This article is only relevant for a minority of users performing high-throughput qPCR analysis (tens of thousands of data points per experiment). It's also restricted to users with at least moderate IT skills and administrator rights on the computer running qbase+.
Despite recent optimizations in qbase+’s calculation engine, some very big experiments may consume more memory than what is made available to qbase+ by default. This will result in swapping to a hard disk drive, thereby significantly affecting the performance of qbase+. Larger experiments can still be properly processed by changeing the amount of memory that may be allocated to qbase+ in a so called ini-file.
For both Linux, Windows and Mac, we recommend to modify the qbase+.ini file to take advantage of more RAM memory (limited to around 1.5 Gb in a 32-bit environment). Open the file with a text editor, locate the line with “-Xmx1024m” and modify the number and unit depending on the amount of RAM available (e.g. “-Xmx3g” on a system with 4 Gb RAM, or “-Xmx7g” on a system with 8 Gb of RAM; general guideline: 1 Gb (g) less than the amount of RAM in Gb; make sure there is no space in between Xmx and the number). These settings need to be updated with every new installation of qbase+. On most systems, administrator rights are required to update these settings.