Does the statistical wizard perform a log transformation?
Yes. As gene expression levels (NRQs) are typically log-normally distributed, all statistical calculations are done on the log transformed CNRQ values.
For easy interpretation of the statistical results, values are re-transformed to linear scale by taking the anti-logarithm. The statistical results are based on the rescaled results, hence, the mean values per subgroup (as well as their respective 95% confidence intervals) will change in function of the target scaling option selected in the Project explorer tree (Settings > Calculation parameters > Target scaling).
Of note, rescaling has no effect on the p-values and fold changes (ratio).